The Effect of Bryonia alba root Extracts on Exogenous and Endogenous Oxidative DNA Damage in Human Lymphocytes
Armen K. Nersesyan
Cancer Research Center, Yerevan, Armenia and Rowett Research Institute, Aberdeen, UK
Corresponding author: Armen K. Nersesyan, Ph.D., D.Sc.
Department of Genetics and Cytology,
State University, 1 Alex Manoukian St,
Yerevan 25, Armenia
Telephone: +3741 552352
Fax number: +3741 286922
E-mail adress: genetik@ysu.am
CEJOEM 2001, Vol.7. Nos.3-4.:209-216
Key words:
Oxitative DNA damage, comet assay, “Loshtak” preparation, chemoprevention
Abbreviations:
LP = “Loshtak” preparation
AE = aqueous extract
ME = methanol extract
FPG = formamidopyrimidine glycolyse
Abstract:
The effect of aqueous and methanol extracts of “Loshtak” preparation (purified Bryonia alba
roots) on exogenous and endogenous oxidative DNA damage was studied on human lymphocytes using the
comet assay with endonuclease III and formamidopyrimidine DNA glycosylase. Extracts of Loshtak
lack ability to protect human cells against exogenous DNA oxidative damage induced by H2O2 but
protect them from the endogenous one. The ability of Loshtak preparation to protect human cells
against endogenous oxidative DNA damage adds a new beneficial property to other effects (e. g.,
lack of genotoxic and carcinogenic activities, antimutagenic effect) of it.
Received: 11 February 2001
Accepted: 11 March 2001
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