Effect of Di-2-ethylhexyl Phthalate on Renal Cytochrome P450 Monooxygenases in
Surgically Evoked Subchronic Renal Failure of Rats
Etelka Kertai1, Judit Kovács2, Gábor
Hollósi3, Zoltán Trestyánszky4, and Vince
Varga3, 5
1 Department of Pharmaceutical Technology
2 Institute of Pathology
3 Department of Animal Anatomy and Physiology
4 Laboratory of Central Pharmacy, University of Debrecen, Debrecen, Hungary
5 Tampere Brain Research Center, University of Tampere Medical School, Tampere, Finland
Corresponding author: Assoc. Prof. Gábor Hollósi
University of Debrecen,
Department of Animal Anatomy and Physiology
H-4010 Debrecen
P.O. Box 18
Hungary
Telephone: (+36) 52-512 900 / Ext. 2337
E-mail:
hollosig@delfin.klte.hu
CEJOEM 2000, Vol.6. No.1.:50-60
Key words:
Subtotal nephrectomy, remnant kidneys, DEHP-treatment, total cytochrome P450
and b5 levels, rat
Abstract:
The effects of di-2-ethylhexyl phthalate (DEHP) were studied on the remnant
kidneys of albino rats during subchronic renal failure (SCRF) induced by subtotal
nephrectomy. Serum creatinine and urea increased significantly already on the seventh
day of DEHP treatment compared with control groups. The amount of creatinine was 3.23
mg/dL and that of urea 100.8 mg/dL (457% and 240% increment, respectively) on the 21st
day of investigation. Changes in the quantity of microsomal total cytochrome P450 and c
ytochrome b5 were also determined, and histological studies undertaken. Both the renal
microsomal P450 and b5 content increased in the remnant kidneys following the exposure
to DEHP, suggesting an induction of enzymes. The elevation of cytochrome P450 level
preceded that of the b5 and reached a higher extent at all times during the investigation.
Microanatomically, the cortex showed hypertrophy, but the medulla was little affected.
A great hypertrophy of glomeruli was observed. Proximal tubular epithelium showed
intracellular vacuolization and degenerative changes.
Received: 08 May 2000
Accepted: 15 June 2000
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