Impact of Different Dose and Size of Industrial Fibrous Dusts on TNF-a
and IFN-g Release
Alzbeta Kaiglová, and Maria Hurbánková
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic
Corresponding author: Alzbeta Kaiglová
Institute of Preventive and Clinical Medicine
Limbová 14, 833 01 Bratislava, Slovak Republic
Tel.: +421-7-376 006
Fax: +421-7-373 906
E-mail: kaigl@upkm.sanet.sk
CEJOEM 1997, Vol.3. No.4.:309-313
Key words:
Tumor necrosis factor-a, interferon-g, amosite fibers, wollastonite
fibers, broncho-alveolar lavage.
Abbreviations:
AM = Alveolar macrophage
BAL = Bronchoalveolar lavage
HRP = Horse radish peroxidase
IFN-g= Interferon-gamma
TNF-a = Tumor necrosis factor-alpha
Abstract:
This study was designed to investigate the changes of TNF-a (tumor
necrosis factor- a) and IFN-g (interferon-g) release by lavage leukocytes
after exposure to thin short and long amosite and wollastonite fibers in
subchronic phase (3 months after the first instillation). Two different
doses were used – 4 and 10 mg/animal. Amphibole asbestos – amosite and
wollastonite fibers belong to naturally occurring inorganic fibers, and
wollastonite is used as a substitute for asbestos. TNF-a and IFN-g are
thought to play an essential role in lung defense against inhaled particles.
Generally, inflammation and lung tissue injury are associated with increased
cytokine levels in the acute phase. Fiber suspensions were carefully administered
intratracheally to W-rats once a week for both 4 and 10 weeks (1 mg/animal/week).
Bronchoalveolar lavage was performed 3 months after the first instillation,
and the cytokine release was evaluated from the isolated lavage leukocytes.
The results of our experiment showed that only a high dose (10 mg/animal)
of long amosite fibers statistically significantly influenced (decreased)
the levels of cytokines TNF-a and IFN-g. The levels of these parameters
were not significantly changed after exposure to other fiber samples, in
comparison with the control. We suppose that the high concentration of
long amosite fibers may lead to the immunosupression of defense mechanism
factors, and contribute to the development of pathological processes.
Received: 24 November 1997
Accepted: 17 December 1997
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Posted: 16 November 1998